[30 µl] $495.00
catalog number: 26906
gene symbol: gnas
description: anti-active gαs-gtp mouse monoclonal antibody
background: heterotrimeric g proteins are critical cellular signal transducers. gαsrepresents one sub-family of g proteins that could mediate the stimulation of adenylyl cyclases.
immunogen: recombinant full length protein of active gαs
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active gαs antibody recognizes active gαs of vertebrates
storage conditions: store at -20°c. avoid freeze / thaw cycles
download datasheet:
immunoprecipitation/western blot:
gαs activation assay. purified gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with gdp (lane 2) or gtpγs (lane 3). immunoprecipitation was done with the anti-active gαs monoclonal antibody (cat. no. 26906). immunoblot was with an anti- gαs monoclonal antibody (cat. no. 26006).
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publications:
1.
am j physiol endocrinol metab. 2012 apr 1;302(7):e747-58
2.
j clin endocrinol metab. 2012 oct;97(10):e1851-61
3.
endocrinology. 2012 apr;153(4):1915-24
4.
mol cell endocrinol. 2012 oct 15;362(1-2):157-64
5.
steroids volume 81, march 2014, pages 36–42
6.
hum. mol. genet. (2014)
武汉纽斯特生物技术有限公司
18972339958
中国 武汉